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aad dual staining  (Elabscience Biotechnology)


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    Elabscience Biotechnology aad dual staining
    Aad Dual Staining, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aad dual staining/product/Elabscience Biotechnology
    Average 95 stars, based on 107 article reviews
    aad dual staining - by Bioz Stars, 2026-05
    95/100 stars

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    Cytotoxicity and cell death measurements after Hypnea musciformis extract (HME) treatment. Dose-dependent inhibition of HME against A Ls174 intestinal and B HepG2 liver cancer cell proliferation. IC 50 values are indicated for each cell line. Cytotoxicity was evaluated using XTT assay. Each point represents mean value ± SD. C Flow cytometry analysis of Ls174 cells stained with Annexin V-PE and 7-AAD, highlighting the percentage of viable, early and late apoptotic and necrotic cells after treatment with HME for 0.5, 1, 2 4, 8, 16 and 24 h, respectively. Each bar represents mean value ± SD. Dashed line demonstrates the change of apoptotic cells as percentage compared to the untreated control. D Same as in C but for HepG2 cells. E Effect of HME on mitochondrial membrane potential (MMP) in Ls174 cells. Each bar represents mean value ± SD. Line demonstrates the change of MMP as percentage compared to the untreated control. F Same as in E but for HepG2 cells. Asterisk marks statistical significance ( p < 0.05) in all panels

    Journal: Human Genomics

    Article Title: Transcriptome and proteome analysis reveals the anti-cancer properties of Hypnea musciformis marine macroalga extract in liver and intestinal cancer cells

    doi: 10.1186/s40246-023-00517-0

    Figure Lengend Snippet: Cytotoxicity and cell death measurements after Hypnea musciformis extract (HME) treatment. Dose-dependent inhibition of HME against A Ls174 intestinal and B HepG2 liver cancer cell proliferation. IC 50 values are indicated for each cell line. Cytotoxicity was evaluated using XTT assay. Each point represents mean value ± SD. C Flow cytometry analysis of Ls174 cells stained with Annexin V-PE and 7-AAD, highlighting the percentage of viable, early and late apoptotic and necrotic cells after treatment with HME for 0.5, 1, 2 4, 8, 16 and 24 h, respectively. Each bar represents mean value ± SD. Dashed line demonstrates the change of apoptotic cells as percentage compared to the untreated control. D Same as in C but for HepG2 cells. E Effect of HME on mitochondrial membrane potential (MMP) in Ls174 cells. Each bar represents mean value ± SD. Line demonstrates the change of MMP as percentage compared to the untreated control. F Same as in E but for HepG2 cells. Asterisk marks statistical significance ( p < 0.05) in all panels

    Article Snippet: The HME-induced cell death type, including both apoptosis and necrosis, was assessed by flow cytometry using the dual staining Annexin V-Phycoerythrin (Annexin V-PE) and 7-Amino-actinomycin D (7-AAD) apoptosis detection kit (BD Pharmingen™, San Diego, CA, USA) according to the manufacturer’s instructions.

    Techniques: Inhibition, XTT Assay, Flow Cytometry, Staining